snail (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
snail
Snail, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+snail/Snail+Rabbit+mAb/pmc13011209-84-66-68
Average 97 stars, based on 2192 article reviews
Snail, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+snail/Snail+Rabbit+mAb/pmc13011209-84-66-68
Average 97 stars, based on 2192 article reviews
snail - by Bioz Stars,
2026-09
97/100 stars
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Saline:Article Title: NNMT inhibition counteracts tubular senescence and fibrosis in early stages of chronic kidney disease. Article Snippet: Protein extracts were denatured in LDS Sample Buffer (Thermo Fisher #NP0007) and Sample Reducing Agent (Thermo Fisher #NP0009) at 70◦C for 10 min 10–20 μg of protein was separated by SDS-PAGE on 4–12% gradient gels (Thermo Fisher #NP0336BOX and #WG1403BOX) with MES SDS Running Buffer (Thermo Fisher #NP0002) and transferred onto PVDF membranes using an iBlot 2 Gel Transfer Device and appropriate transfer stacks (Thermo Fisher #IB24001). .. Membranes were blocked in 5% non-fat dry milk 26 Cell Reports 45, 116823, January 27, 2026 in Tris-buffered saline-Tween 20 (TBST, Thermo Fisher #28360), and incubated overnight at 4◦C with primary antibodies anti-p21 (1:500, Abcam #107099, kidney tissue), anti-p21 (1:500, Abcam #188224, cells), anti-NNMT (1:500, Abcam #119758, kidney tissue), anti-NNMT (1:500, Proteintech #15123-1-AP, cells), anti α-SMA (1:1000, Cell Signaling #19245, kidney tissue and cells), anti-Twist (1:1000, Cell Signaling #90445, cells), Incubation:Article Title: NNMT inhibition counteracts tubular senescence and fibrosis in early stages of chronic kidney disease. Article Snippet: Protein extracts were denatured in LDS Sample Buffer (Thermo Fisher #NP0007) and Sample Reducing Agent (Thermo Fisher #NP0009) at 70◦C for 10 min 10–20 μg of protein was separated by SDS-PAGE on 4–12% gradient gels (Thermo Fisher #NP0336BOX and #WG1403BOX) with MES SDS Running Buffer (Thermo Fisher #NP0002) and transferred onto PVDF membranes using an iBlot 2 Gel Transfer Device and appropriate transfer stacks (Thermo Fisher #IB24001). .. Membranes were blocked in 5% non-fat dry milk 26 Cell Reports 45, 116823, January 27, 2026 in Tris-buffered saline-Tween 20 (TBST, Thermo Fisher #28360), and incubated overnight at 4◦C with primary antibodies anti-p21 (1:500, Abcam #107099, kidney tissue), anti-p21 (1:500, Abcam #188224, cells), anti-NNMT (1:500, Abcam #119758, kidney tissue), anti-NNMT (1:500, Proteintech #15123-1-AP, cells), anti α-SMA (1:1000, Cell Signaling #19245, kidney tissue and cells), anti-Twist (1:1000, Cell Signaling #90445, cells), Article Title: LncRNA VCAN‐AS1 Sponges miR‐374c‐3p to Promote Proliferation, Invasion, Migration, and EMT in Thyroid Cancer Article Snippet: .. After blocking in TBST containing 5% skim milk for 2 h at room temperature, the membrane was incubated with primary antibodies at 4°C overnight and then cultured with horseradish peroxidase‐conjugated secondary antibodies (anti‐mouse IgG, #7076, or anti‐rabbit IgG, #7074; 1:5000, Cell Signaling Technology, USA) in 37°C for 2 h. The primary antibodies were as follows: anti‐E‐cadherin (1:1000, 20871‐1‐AP, proteintech), anti‐N‐cadherin (1:1000, 13116T, Cell Signaling Technology), anti‐vimentin (1:1000, 60330‐1‐LG, proteintech), anti‐slug (1:1000, ab51772, Abcam), Blocking Assay:Article Title: LncRNA VCAN‐AS1 Sponges miR‐374c‐3p to Promote Proliferation, Invasion, Migration, and EMT in Thyroid Cancer Article Snippet: .. After blocking in TBST containing 5% skim milk for 2 h at room temperature, the membrane was incubated with primary antibodies at 4°C overnight and then cultured with horseradish peroxidase‐conjugated secondary antibodies (anti‐mouse IgG, #7076, or anti‐rabbit IgG, #7074; 1:5000, Cell Signaling Technology, USA) in 37°C for 2 h. The primary antibodies were as follows: anti‐E‐cadherin (1:1000, 20871‐1‐AP, proteintech), anti‐N‐cadherin (1:1000, 13116T, Cell Signaling Technology), anti‐vimentin (1:1000, 60330‐1‐LG, proteintech), anti‐slug (1:1000, ab51772, Abcam), Membrane:Article Title: LncRNA VCAN‐AS1 Sponges miR‐374c‐3p to Promote Proliferation, Invasion, Migration, and EMT in Thyroid Cancer Article Snippet: .. After blocking in TBST containing 5% skim milk for 2 h at room temperature, the membrane was incubated with primary antibodies at 4°C overnight and then cultured with horseradish peroxidase‐conjugated secondary antibodies (anti‐mouse IgG, #7076, or anti‐rabbit IgG, #7074; 1:5000, Cell Signaling Technology, USA) in 37°C for 2 h. The primary antibodies were as follows: anti‐E‐cadherin (1:1000, 20871‐1‐AP, proteintech), anti‐N‐cadherin (1:1000, 13116T, Cell Signaling Technology), anti‐vimentin (1:1000, 60330‐1‐LG, proteintech), anti‐slug (1:1000, ab51772, Abcam), Cell Culture:Article Title: LncRNA VCAN‐AS1 Sponges miR‐374c‐3p to Promote Proliferation, Invasion, Migration, and EMT in Thyroid Cancer Article Snippet: .. After blocking in TBST containing 5% skim milk for 2 h at room temperature, the membrane was incubated with primary antibodies at 4°C overnight and then cultured with horseradish peroxidase‐conjugated secondary antibodies (anti‐mouse IgG, #7076, or anti‐rabbit IgG, #7074; 1:5000, Cell Signaling Technology, USA) in 37°C for 2 h. The primary antibodies were as follows: anti‐E‐cadherin (1:1000, 20871‐1‐AP, proteintech), anti‐N‐cadherin (1:1000, 13116T, Cell Signaling Technology), anti‐vimentin (1:1000, 60330‐1‐LG, proteintech), anti‐slug (1:1000, ab51772, Abcam), |